SIGMA-ALDRICH N3520-10X1L Nutrient Mixture Ham F-12

( Brand: Sigma-aldrich ), ( Manufacturer Part Number: N3520-10X1L ), ( Country/region Of Manufacture: United States )

Review SIGMA ALDRICH N3520-10x1 Nutrient Mixture F-12 Ham

Sigma-Aldrich's N3520-10x1L F-12 Ham Nutrient Mixture is a premium quality, ready-to-use supplement designed for use in cell culture applications, specifically for growing hamster kidney (BHK-21) cells. This nutrient mixture is carefully formulated to provide optimal growth conditions for these cells, ensuring maximum cell health and productivity.

The N3520-10x1L F-12 Ham Nutrient Mixture is based on the F-12 Kaighn's Modification of Eagle's Minimal Essential Medium. It contains essential nutrients, vitamins, and minerals required for cell growth and metabolism, including amino acids, vitamins, inorganic salts, and trace elements. The medium also includes sodium pyruvate and L-glutamine, which are important energy sources and metabolic precursors for cells.

This nutrient mixture is free from antibiotics and antimycotics, making it an excellent choice for researchers working with sensitive cell lines or conducting experiments that require antibiotic-free conditions. It is also sterile-filtered to ensure that it is free from contaminants and can be directly added to cell culture flasks or bioreactors without further sterilization.

The N3520-10x1L F-12 Ham Nutrient Mixture is supplied in a convenient 1-liter bottle, with a concentration of 10x. This means that one liter of the stock solution can be diluted with nine liters of sterile water to make a final volume of ten liters of complete medium. The bottle is also fitted with a tamper-evident seal to ensure the integrity of the product and prevent contamination.

In summary, Sigma-Aldrich's N3520-10x1L F-12 Ham Nutrient Mixture is a high-quality, antibiotic-free supplement specifically formulated for growing hamster kidney (BHK-21) cells. It contains a complete set of essential nutrients, vitamins, and minerals required for cell growth and metabolism, and is conveniently supplied in a sterile, 1-liter bottle with a tamper-evident seal.

Sigma-Aldrich's N3520-10x1L F-12 Ham's Nutrient Mixture is a widely used medium for growing cells in laboratory conditions. Here are some pros and cons to help you decide if this product is suitable for your research needs:

1. Widely accepted: F-12 Ham's Nutrient Mixture is a well-established medium for growing various cell lines, particularly those of neural and epithelial origin.

2. Convenience: Pre-prepared media like the N3520-10x1L save time and effort as they eliminate the need to prepare media from individual components.

3. Consistent quality: Sigma-Aldrich is a reputable supplier known for its high-quality products, which can ensure consistent and reliable results.

4. Long shelf life: This medium has a long shelf life when stored properly, reducing the need for frequent replenishment.

5. Versatility: F-12 Ham's Nutrient Mixture can be used for both adherent and suspension cell cultures.

1. Cost: Pre-prepared media like the N3520-10x1L can be more expensive compared to preparing media from individual components.

2. Limited customization: Pre-prepared media come with a fixed composition, which may not meet the specific requirements of certain cell lines or experiments.

3. Compatibility: It is essential to ensure that this medium is compatible with the specific cell line or experiment being carried out to avoid any potential negative effects.

In conclusion, the Sigma-Aldrich N3520-10x1L F-12 Ham's Nutrient Mixture offers convenience, consistency, and versatility for growing various cell lines, but it may come with a higher cost compared to preparing media from individual components. Researchers should carefully consider the specific requirements of their experiment and ensure compatibility before purchasing this medium.

If you are working with commonly used cell lines, particularly those of neural and epithelial origin, and require a reliable and convenient media source, the Sigma-Aldrich N3520-10x1L F-12 Ham's Nutrient Mixture could be an excellent choice. However, if you have specific requirements or are working on a tight budget, preparing media from individual components might be a more cost-effective and customizable option.

Details:

Sigma N3520-10X1 Nutrient mixture F-12 Ham Brand new.

part #: n352010x1l price

• $50.00

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• brand: Sigma-aldrich
• manufacturer part number: N3520-10X1L
• country/region of manufacture: United States

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