MEDCHEM EXPRESS HY-126561 Mce Celltracker Fluorescent Probe Powder Cmfda

( Brand: Medchem Express ), ( Type: Biological Assays ), ( Unit Type: 1 Vial ), ( Manufacturer Part Number: HY-126561 ), ( Unit Quantity: 1 Mg ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory ), ( California Prop 65 Warning: For Laboratory Research Use Only. Not Intended For Diagnostic Or Clinical Use. )

Review MEDCHEM EXPRESS Mce Celltracker Fluorescent Probe Green Cmfda Mg Powder

Product Name: MedChemHy-126561, MCE CellTracker CMFDA Powder

This MedChemHy-126561 product, also known as MCE CellTracker CMFDA Powder, is a versatile and essential tool in the field of cell biology research. It is a fluorescent probe specifically designed for the labeling of living cells, enabling researchers to visualize and track cellular processes in real-time.

The primary function of this fluorescent probe is to label living cells with the 5-chloromethylfluorescein diacetate (CMFDA) derivative. Once taken up by cells through the plasma membrane, the probe is hydrolyzed intracellularly by cellular esterases to release the fluorescent 5-chloromethylfluorescein (CMF) molecule. This fluorescent molecule emits bright green fluorescence when excited with light in the blue wavelength range (around 490nm).

The high specificity and sensitivity of the probe make it an excellent choice for various applications, such as cell tracing, cell cycle analysis, and cell sorting. With its ability to label living cells, it is particularly useful for studying dynamic cellular processes, such as cell migration, proliferation, and differentiation.

The powder form of this product allows for easy handling and storage, making it a convenient choice for researchers. It is recommended to dissolve the powder in an appropriate solvent, such as DMSO or PBS, before use. The final concentration of the working solution will depend on the specific requirements of the research application.

The MCE CellTracker CMFDA Powder is produced under stringent quality control conditions, ensuring its purity and consistency. It is supplied in a small, resealable bag to maintain its stability and prevent contamination. This product is compatible with various cell types and can be used in a wide range of experimental conditions.

In summary, the MedChemHy-126561 or MCE CellTracker CMFDA Powder is a valuable research tool for cell biologists, enabling them to visualize and study living cells in real-time with high specificity and sensitivity. Its ease of use, versatility, and excellent fluorescent properties make it an indispensable component of any cell biology laboratory.

Medchem Hy-126561, also known as CMFDA or Calcium Amylose Fuchsin Acetate, is a fluorescent probe commonly used in cell biology research for the detection of intracellular calcium ion (Ca2 ) changes. Here are some pros and cons of buying this powder for your experiments:

1. High sensitivity and specificity: The probe is highly sensitive to Ca2 and selectively binds to it, making it an effective tool for monitoring calcium signaling in cells.

2. Bright and stable fluorescence: The fluorescence emitted by the probe is bright and stable, allowing for easy detection and quantification of calcium ion changes.

3. Wide dynamic range: The probe can detect a wide range of calcium ion concentrations, making it suitable for studying both small and large changes in intracellular Ca2 levels.

4. Easy to use: The probe is relatively easy to use, requiring only a short incubation time and minimal washing steps before imaging or analysis.

5. Cost-effective: The powder form of the probe is typically less expensive than pre-prepared solutions or kits.

1. Toxicity: The probe may be toxic to some cell types, especially at high concentrations. It is important to optimize the working concentration for each specific cell type to minimize toxicity and maintain cell viability.

2. Potential for photobleaching: The fluorescence of the probe may be susceptible to photobleaching, which can reduce the signal intensity and limit the amount of data that can be collected.

3. Requires special handling: The probe should be handled with care to avoid contamination and ensure optimal performance. It should be stored in the dark at low temperatures and protected from light during use.

4. May require additional reagents: Depending on the specific application, additional reagents such as calcium ion chelators or indicator dyes may be required to optimize the signal and distinguish between different types of calcium ion changes.

In conclusion, Medchem Hy-126561 (CMFDA powder) is a valuable tool for monitoring intracellular calcium ion changes in research applications. Its high sensitivity, bright fluorescence, and wide dynamic range make it an effective probe for studying calcium signaling. However, it requires careful handling and optimization of working concentrations to minimize toxicity and ensure optimal performance. Overall, the benefits of using this probe outweigh the potential drawbacks, making it a recommended choice for researchers in the field of cell biology and calcium signaling.

Details:

Excitation Wave length Range485 NM, Dye Green CMFDA, Quantity 1 mg, CellTracker CMFDA Dye, Emission514 Form Dry Powder, Product Line CMFDA, CellTracker, Reagent Type Cell Tracker Compounds, Cell Labeling Reagents, Shipping Condition Room Temperature, Molecular Weight464.85, Type Dye, Sub Cellular Localization Cytoplasm.: mg.

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• brand: Medchem Express
• type: Biological Assays
• unit type: 1 Vial
• manufacturer part number: HY-126561
• unit quantity: 1 Mg
• country of origin: United States
• intended use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory
• california prop 65 warning: For Laboratory Research Use Only. Not Intended For Diagnostic Or Clinical Use.

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