INVITROGEN C34552 Celltracker Fluorescent Probe Cmtpx 50

( Brand: Invitrogen ), ( Type: Biological Assays ), ( Unit Type: 1 Vial ), ( Manufacturer Part Number: C34552 ), ( Unit Quantity: 50 G ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory ), ( California Prop 65 Warning: For Laboratory Research Use Only. Not Intended For Diagnostic Or Clinical Use. )

Review INVITROGEN Celltracker Fluorescent Probe Red Cmtpx 50

The Invitrogen C34552 CellTracker CM-TPX-50 Green Fluorescent Probe is a versatile tool for labeling living cells with green fluorescence. This probe is part of the CellTracker dye family, which is widely used in various research applications, including cell tracking, cell sorting, and flow cytometry.

The CM-TPX-50 Green dye specifically targets intracellular acidic compartments such as lysosomes and endosomes. When added to cells, this probe is taken up by the cells and localizes to these organelles, allowing for visualization and analysis of their dynamics. The green fluorescence emitted by the probe can be easily distinguished from other fluorescent labels, making it an ideal choice for co-localization studies.

The C34552 CellTracker CM-TPX-50 Green probe comes as a powder in a vial, and it requires dilution in a suitable solvent before use. The recommended solvent is a cell culture medium, such as Dulbecco's Modified Eagle Medium (DMEM) or RPMI 1640. The probe is light-sensitive, so it is important to protect it from bright light during preparation and application.

The working concentration of the probe varies depending on the specific application and the desired level of labeling. For general cell labeling, a concentration of 1-5 M is typically used. For more intense labeling, up to 20 M can be used. It is important to optimize the concentration for each experiment, as excessive labeling can affect cell function and viability.

The probe labeling process is typically carried out at 37 C for 15-30 minutes. During this time, the probe is taken up by the cells and localizes to the acidic compartments. After labeling, the cells are washed extensively to remove any unbound probe and are ready for further analysis.

In summary, the Invitrogen C34552 CellTracker CM-TPX-50 Green Fluorescent Probe is a powerful tool for labeling and visualizing intracellular acidic compartments in living cells. Its green fluorescence allows for easy distinction from other labels and makes it an ideal choice for co-localization studies. The probe is light-sensitive and requires dilution in a suitable solvent before use, and the optimal working concentration and labeling time should be determined experimentally.

The Invitrogen C34552 CellTracker CMTMX-50 (also known as CMTMX Red) is a fluorescent probe used for labeling living cells for various applications, including cell tracking, lineage analysis, and flow cytometry. Here are some pros and cons of buying this product:

1. Versatile: This probe can be used to label various types of cells, including hematopoietic, neural, and epithelial cells.

2. Long-lasting: The probe is designed to be stable in living cells for extended periods of time, making it ideal for long-term cell tracking studies.

3. Bright fluorescence: The CMTMX Red probe emits a bright and distinct red fluorescence, making it easy to detect and distinguish labeled cells from unlabeled ones.

4. Low toxicity: The probe is relatively non-toxic to cells, making it suitable for use in a wide range of experimental conditions.

5. Easy to use: The probe is simple to use and can be easily dissolved in culture medium or other solvents for cell labeling.

1. Expensive: Compared to other fluorescent probes, the CMTMX Red probe can be quite pricey, especially when considering the small amount of product that comes in each kit.

2. Requires careful handling: The probe should be handled with care to avoid photobleaching and other forms of damage that can affect its fluorescence.

3. Specific application: While the probe is versatile, it is primarily used for cell tracking and lineage analysis applications. If you are looking for a probe for other applications, there may be better options available.

4. Potential for non-specific labeling: Like all fluorescent probes, there is a risk of non-specific labeling when using the CMTMX Red probe. Careful experimental design and control experiments are essential to minimize this risk.

In conclusion, the Invitrogen C34552 CellTracker CMTMX-50 (CMTMX Red) is a high-quality fluorescent probe that offers excellent labeling performance for a range of cell tracking applications. However, it comes with a higher price tag and requires careful handling. Ultimately, the decision to purchase this probe depends on the specific requirements of your experiment and your research budget. If you need to label a large number of cells or require a more cost-effective option, you may want to consider alternative fluorescent probes. But if you need a reliable and brightly labeled probe for long-term cell tracking studies, then the CMTMX Red probe is an excellent choice.

Details:

The CellTracker fluorescent probes are available in a range of colors to match instrument lasers and filters, accommodate co-staining with antibodies or other cell analysis. Low does not affect viability or proliferation. Excitation Wave length Range577 NM, Dye Red CMTPX, Quantity 1 x 50 g, CellTracker CMTPX Dye, 20 Emission602 Form Dry Powder, Product , Reagent Type Cell Tracker Compounds, Cell Labeling Reagents. The CellTracker dyes are retained with fixation and demobilization, enabling their use antibodies for fluorescence amnestying applications.

Features of the CellTracker dyes include: Easy to use remove culture media, add dye, incubate 15-45 minutes, and image cells. Cell Versatility works with cells in suspension and adherent. The probes are transferred to daughter cells, but not adjacent cells in a population. CellTracker is a fluorescent dye well suited for monitoring cell movement or location.

part #: c34552 price

• $28.00

specifications quakedental:

• brand: Invitrogen
• type: Biological Assays
• unit type: 1 Vial
• manufacturer part number: C34552
• unit quantity: 50 G
• country of origin: United States
• intended use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory
• california prop 65 warning: For Laboratory Research Use Only. Not Intended For Diagnostic Or Clinical Use.

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Invitrogen C34552 Is Similar To:

• Invitrogen C34551 Celltracker Fluorescent Probe Orange Cmra 50 G (88.9% similar) The celltracker fluorescent probes are available in a range of colors to match instrument lasers and filters, accommodate co-staining with antibodies or other cell analysis. Celltracker is a fluorescent dye well suited for monitoring cell movement or location. The probes are transferred to...(May 3rd, 2026)