PHARMACIA 9338004 Ge Phenyl Superose Fplc Column

( Brand: Pharmacia ), ( Manufacturer Part Number: 9338004 )

Review PHARMACIA Ge Phenyl Superose Fplc Column

The Pharmacia 9338004 GE Phenyl Sepharose HP FPLC Column is a high-performance, size-exclusion chromatography (SEC) column designed for the purification and separation of proteins and other biomolecules. This particular column is packed with Phenyl Sepharose HP resin, which is a hydrophobic, cross-linked agarose bead matrix that possesses phenyl group functionalities. These functionalities interact with hydrophobic regions of proteins, allowing for effective binding and separation based on hydrophobic interaction chromatography (HIC).

The column measures 10 cm in length and 2.5 cm in diameter, providing a total packing volume of approximately 120 ml. The resin beads have an average particle size of 40-160 m, ensuring good flow characteristics and high resolution separations. The column is specifically designed for use with FPLC (Fast Protein Liquid Chromatography) systems, which allow for efficient and automated separations.

The Phenyl Sepharose HP FPLC Column is an excellent choice for a wide range of applications, including the purification of recombinant and native proteins, the removal of contaminants such as lipids and nucleic acids, and the preparation of protein complexes for structural and functional studies. The column's hydrophobic interaction mechanism allows for the separation of proteins based on subtle differences in their hydrophobic properties, making it a powerful tool for the characterization of complex protein mixtures.

The column is supplied in a protective jacket, which helps to maintain the column's integrity during storage and transport. It is recommended that the column be stored at room temperature and that the mobile phase be filtered prior to use to ensure the best possible separation results.

In summary, the Pharmacia 9338004 GE Phenyl Sepharose HP FPLC Column is a versatile and powerful tool for the purification and separation of proteins and other biomolecules. Its hydrophobic interaction mechanism, combined with its high resolution and efficient separations, makes it an indispensable component in many research and industrial laboratories.

Pros and Cons of Buying Pharmacia 9338004 Ge Phenyl Superose FPLC Column

The Pharmacia 9338004 Ge Phenyl Superose FPLC column is a popular choice for protein purification using FPLC (Fast Protein Liquid Chromatography). In this analysis, we will discuss the advantages and disadvantages of purchasing this column to help researchers make informed decisions.

1. High Resolution: This column offers excellent resolution and separation efficiency, making it suitable for purifying proteins with complex mixtures or low abundance.

2. Wide Protein Range: It can handle a wide range of protein sizes (from small peptides to large proteins) and can separate proteins based on charge, hydrophobicity, and size.

3. Reusable: The column can be reused multiple times, reducing the overall cost per purification.

4. Easy to Use: The column comes pre-equipped with a flow-through design, making it user-friendly and easy to set up.

5. Consistent Results: The consistent and reproducible results make it a popular choice for both research and industrial applications.

1. Expensive: The initial cost of purchasing a column can be high, which may deter some researchers or smaller laboratories.

2. Requires Specific Equipment: To use the column, researchers need access to an FPLC system, which can be expensive and not readily available in all laboratories.

3. Time-consuming: The purification process using this column can take several hours, which may not be suitable for researchers with tight deadlines.

4. Limited Capacity: The column has a relatively low capacity, which may require users to perform multiple purifications for larger protein quantities.

The Pharmacia 9338004 Ge Phenyl Superose FPLC column is a high-performance tool for protein purification, providing excellent resolution, wide protein range, and consistent results. However, its high cost, requirement for specific equipment, time-consuming process, and limited capacity may be disadvantages for some researchers. Ultimately, the decision to purchase this column depends on the specific needs, resources, and goals of the research project.

For researchers working on projects that require high-resolution protein purification and have access to the necessary equipment and budget, the Pharmacia 9338004 Ge Phenyl Superose FPLC column is a strong recommendation. For researchers with limited resources, alternative methods such as column chromatography or affinity purification may be more cost-effective options. It's crucial to consider the specific requirements of the project and the available resources before making a decision.

Details:

This listing is for a Pharmacia Phenyl Superose FPLC column protein chromatography. Comes as pictured with attached tubing.

part #: 9338004 price

• $99.99

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• brand: Pharmacia
• manufacturer part number: 9338004

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