


( Brand: Applied Biosystems ), ( Part Type: Box Starter ), ( Manufacturer Part Number: 4316823 ), ( Intended Use/discipline: Biological Laboratory )
The Applied Biosystems 4316823 Box FG Starter Kit is specifically designed for use with the Applied Biosystems 2720 Thermal Cycler, a versatile and reliable instrument that delivers consistent PCR results. This essential starter kit includes all necessary reagents and consumables for performing polymerase chain reaction (PCR) experiments.
The Box FG Starter Kit contains 20 preparations of each of the following Master Mixes, sufficient for approximately 20 reactions per preparation:1. GoTaq Green Master Mix: Ideal for general PCR applications, this mix features a high-fidelity Taq polymerase that delivers reproducible results, even with difficult templates.
2. GoTaq Flexi Buffer System: This versatile buffer system enables optimization of PCR conditions for a wide range of templates, offering flexibility to suit specific amplification requirements.
3. GoTaq Q fitted Buffer System: Suitable for Q-Flex Primer-Dependent Nucleotide Incorporation (Quasiphotometric) assays and other high-throughput PCR applications.
Additionally, the kit includes a set of the following reagents:1. 10x PCR Buffer: Helps provide a stable reaction environment, optimizing the pH and buffer concentration for PCR.
2. MgCl2 Solution: Contains magnesium ions that improve enzyme binding and enzyme activity.
3. dNTP Solution: Provides the required deoxynucleotides for DNA synthesis during PCR.
4. GoTaq Enzyme: A high-fidelity DNA polymerase that effectively amplifies genomic DNA and synthetic templates.
5. Mineral Oil: Used to prevent evaporation of PCR reagents and help maintain a consistent reaction temperature.
6. TE Buffer: A versatile buffer that can be used for eluting fragments from agarose gels and as a general-purpose buffer for working with nucleic acids.
The Applied Biosystems 4316823 Box FG Starter Kit provides an excellent starting point for researchers and laboratories seeking to perform PCR using the 2720 Thermal Cycler, allowing them to complete their experiments both efficiently and cost-effectively. With a strong focus on reproducibility, this starter kit ensures that every PCR amplification reaction will yield consistent, reliable results.
The Applied Biosystems 4316823 Box FG Starter Kit is designed for use with the 2720 Thermal Cycler in performing PCR (Polymerase Chain Reaction) experiments. PCR is a widely-used laboratory technique for amplifying specific DNA sequences. Below are some potential pros and cons of purchasing this particular starter kit:
Pros:1. Convenience: The Box FG Starter Kit is designed to provide all the essential reagents and consumables required for running PCR experiments using the 2720 Thermal Cycler. This can save time and resources for scientists and researchers who may not have these reagents readily available in their lab.
2. Quality: Applied Biosystems is a reputable company in the field of life sciences and diagnostics, and their products are known for their high-quality and reliability. The 4316823 Box FG Starter Kit is no exception, providing consistent PCR results for various applications.
3. Flexibility: The kit is designed to be versatile and can be used for various PCR applications, including genotyping, sequencing, and diagnostic assays.
4. Easy-to-use: The Box FG Starter Kit includes detailed instructions, making it a user-friendly option for researchers and scientists who may be new to PCR experiments or the 2720 Thermal Cycler.
Cons:1. Cost: The Box FG Starter Kit can be expensive compared to older or generic PCR reagents. However, the investment may be worthwhile for the convenience and reliability offered.
2. Limited Capacity: The 2720 Thermal Cycler has a relatively small capacity for PCR reactions compared to other thermal cyclers, allowing for only 16 standard 0.2mL thin-walled reaction tubes or 8 vertical 0.2mL PCR strips.
3. Compatibility: The Box FG Starter Kit is specifically designed for use with the Applied Biosystems 2720 Thermal Cycler, which may not be the primary thermal cycler used in all research labs.
In conclusion, the Applied Biosystems 4316823 Box FG Starter Kit can offer convenience, reliability, and flexibility for PCR experiments. However, it comes at a higher cost compared to some other options, and its limited capacity may not be suitable for large-scale research projects. Ultimately, whether or not to purchase this starter kit depends on the specific needs and resources of your research lab.
Recommendation:If your lab relies on PCR experiments and does not have a thermal cycler or the necessary reagents on hand, or if you require a reliable and consistent PCR setup, the Applied Biosystems 4316823 Box FG Starter Kit can be a worthwhile investment. However, if your lab already has a thermal cycler and a sufficient supply of PCR reagents, or if budget is a primary concern, alternative options may be considered. It's essential to assess your lab's specific requirements and financial situation to make an informed decision.
NEW OPEN BOX APPLIED BIOSYSTEMS 4316823 FG Starter Kit for 2720 Thermal Cycler.