
( Brand: Bio-rad ), ( Part Type: Tran Transfer ), ( Manufacturer Part Number: 162-0115 ), ( Country Of Origin: United States )
The Bio-Rad 162-0115 Trans-Blot Nitrocellulose Transfer Membrane 0.45 um is a high-quality, PVDF (polyvinylidene difluoride) membrane designed for use in the transfer of proteins from gel electrophoresis to nitrocellulose or PVDF membranes. This membrane is an essential component in the process of Western blotting, a common laboratory technique used for the identification and quantification of proteins.
The 0.45 um pore size of this membrane allows for efficient transfer of proteins from the gel to the membrane, ensuring a high degree of resolution and clarity in the results. The PVDF material offers several advantages over traditional nitrocellulose membranes, including greater durability, improved binding capacity, and reduced background noise.
Each roll of the Bio-Rad 162-0115 membrane contains 10 sheets, measuring 14.3 cm x 14.3 cm (5.6 inches x 5.6 inches), providing ample material for multiple experiments. The membranes are pre-cut to size, simplifying the setup process and reducing waste.
The membranes are prepared with a uniform surface coating that enhances protein binding and ensures even transfer, which is crucial for accurate and reliable results. Additionally, the membranes are compatible with a wide range of transfer buffer systems and staining methods commonly used in Western blotting.
This Bio-Rad membrane is also easy to handle, with a smooth and flexible surface that can be easily marked for lane alignment and cut to size if needed. The membranes can be stored at room temperature or refrigerated for extended periods prior to use, ensuring that they remain in excellent condition.
In summary, the Bio-Rad 162-0115 Trans-Blot Nitrocellulose Transfer Membrane 0.45 um is a versatile and high-performance membrane that is ideal for use in Western blotting applications. Its uniform pore size, PVDF material, and pre-cut size make it a convenient and reliable choice for researchers and laboratory technicians seeking accurate and efficient protein transfer results.
a. High-quality membrane: Bio-Rad is a reputable brand known for producing high-quality laboratory supplies, including nitrocellulose transfer membranes.
b. Consistent results: This membrane provides reliable and consistent results for various blotting experiments, ensuring accurate data.
c. Easy handling: The 0.45 m pore size is suitable for most protein size ranges, making it a versatile choice for researchers.
d. Large sheet size: The 15.2 cm x 15.2 cm (6 in x 6 in) size allows for transferring larger samples or multiple samples in one blot.
e. Sterile: The membrane is sterilized, reducing the risk of contamination during the transfer process.
2. Cons:a. Cost: Bio-Rad membranes tend to be pricier compared to other brands, which could be a disadvantage for researchers with limited budgets.
b. Limited shelf life: Once opened, the membrane should be used as soon as possible to ensure optimal performance. Unused membranes should be stored in a dry place and away from light to maintain their quality.
c. Requires specific handling: The membrane must be handled correctly during the transfer process to avoid damage and ensure efficient transfer.
Conclusion:The Bio-Rad 162-0115 Trans-Blot Nitrocellulose Transfer Membrane (0.45 um) offers several advantages, such as high-quality results, easy handling, and large sheet size. However, its higher cost and limited shelf life are potential disadvantages. Researchers should consider the benefits and drawbacks of this membrane in relation to their specific needs and budget.
Recommendation:If researchers require reliable and consistent results for their blotting experiments and can afford the higher cost, the Bio-Rad 162-0115 Trans-Blot Nitrocellulose Transfer Membrane (0.45 um) is an excellent choice due to its high-quality and versatility. Researchers on a tight budget may consider exploring alternative brands or membrane types to find a more cost-effective solution while maintaining acceptable performance.
Bio-Rad Trans Blot Nitrocellulose Transfer Membrane 0.45uM Cat# 162-0115 partly used, Comes from a Midwest research university.